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lambda fix ii 129/svj mouse genomic library  (Agilent technologies)


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    Structured Review

    Agilent technologies lambda fix ii 129/svj mouse genomic library
    Lambda Fix Ii 129/Svj Mouse Genomic Library, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lambda+fix+ii+svj/pmc01271812-302-1-8
    Average 90 stars, based on 1 article reviews
    lambda fix ii 129/svj mouse genomic library - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Kidney development in cadherin-6 mutants: delayed mesenchyme-to-epithelial conversion and loss of nephrons.
    Article Snippet: Genomic sequences for Cad-6 were isolated from an isogenic Lambda FIX II SVJ/129 mouse genomic library (Stratagene) screened with the first 651 bp of Cad-6 cDNA previously described (Cho et al., 1998).

    Article Title: Cloning and characterization of the multiple murine homologues of NAIP (neuronal apoptosis inhibitory protein).
    Article Snippet: The spinal muscular atrophies (SMAs), characterized by degeneration of spinal cord motor neurons, are among the most common autosomal recessive disorders.. We have previously reported the characterization of an SMA-associated gene designated NAIP (neuronal apoptosis inhibitory protein).. This gene, which encodes a protein homologous to the baculoviral inhibitor of apoptosis proteins, is deleted in a significant proportion of individuals with type I SMA, is expressed in motor neurons, and inhibits apoptosis both in vitro and in vivo.

    Labeling:

    Article Title: Gene expression profiling in the submandibular gland, stomach, and duodenum of CAVI-deficient mice.
    Article Snippet: Carbonic anhydrase VI (CAVI) is the only secreted isozyme of the a-carbonic anhydrase family, which catalyzes the reversible reaction CO2 þ H2O, HCO 3 þ Hþ.. It appears that CAVI protects teeth and gastrointestinal mucosa by neutralizing excess acidity.. However, the evidence for this physiological function is limited, and CAVI may have additional functions that have yet to be discovered.

    Cloning:

    Article Title: Genomic architecture and transcriptional activation of the mouse and human tumor susceptibility gene TSG101: common types of shorter transcripts are true alternative splice variants.
    Article Snippet: The tsg101 cDNA probe, after sequencing analysis, was subcloned into the pZERO-1 vector (Invitrogen) and used for hybridization and cloning of tsg101 genomic DNA and Northern blot analysis. .. Cloning of the mouse tsg101 gene and a processed pseudogene Initially, we screened a lambda phage mouse genomic library (129/SVJ Lambda FIX II, Stratagene) following the manufacture's protocol with 32P-labeled tsg101 cDNA (Random Priming Kit, Stratagene). ..

    Derivative Assay:

    Article Title: Mesothelin Is Not Required for Normal Mouse Development or Reproduction
    Article Snippet: The immunoglobulin G antibodies (IgGs) from the rabbit antisera were then purified on an immobilized protein A matrix (Pierce Chemical, Rockford, Ill.) following the supplier's instructions. .. A 129 SVJ mouse genomic lambda FIX II phage library (Stratagene, La Jolla, Calif.) was screened with a probe derived from the 5′ end of the m-mesothelin cDNA. .. A clone containing an insert of approximately 12 kb was subcloned in pBluescript II S/K plasmid (Stratagene), and the restriction map of the insert was determined.

    Article Title: Cse1l Is Essential for Early Embryonic Growth and Development
    Article Snippet: PCR-amplified fragments were then cloned in Topo TA cloning vector (Invitrogen, Carlsbad, Calif.), and DNA sequences were determined with an ABI 373 DNA sequencer and Applied Biosystem's Dye terminator kit. .. A 129 SVJ mouse genomic Lambda FIX II phage library (Stratagene, La Jolla, Calif.) was screened with a probe derived from the 3′ end of the Cse1l cDNA. ..

    Article Title: Cse1l Is Essential for Early Embryonic Growth and Development
    Article Snippet: .. A 129 SVJ mouse genomic Lambda FIX II phage library (Stratagene, La Jolla, Calif.) was screened with a probe derived from the 3 end of the Cse1l cDNA. ..

    RNA Extraction:

    Article Title: Genetic ablation of Ptprj, a mouse cancer susceptibility gene, results in normal growth and development and does not predispose to spontaneous tumorigenesis.
    Article Snippet: Ptprj is a ubiquitously expressed murine gene encoding a receptor-type protein tyrosine phosphatase, which has recently been proposed as a candidate gene on the locus Scc1 for colon cancer susceptibility.. It has been demonstrated that PTPRJ, the human homologue of Ptprj, is involved in the control of cell growth and adhesion, being furthermore altered in several types of cancer including mammary, thyroid, lung, colon, and pancreatic cancers.. To investigate the biological functions of Ptprj, we have generated mice deficient in this receptor protein tyrosine phosphatase.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Genetic ablation of Ptprj, a mouse cancer susceptibility gene, results in normal growth and development and does not predispose to spontaneous tumorigenesis.
    Article Snippet: Ptprj is a ubiquitously expressed murine gene encoding a receptor-type protein tyrosine phosphatase, which has recently been proposed as a candidate gene on the locus Scc1 for colon cancer susceptibility.. It has been demonstrated that PTPRJ, the human homologue of Ptprj, is involved in the control of cell growth and adhesion, being furthermore altered in several types of cancer including mammary, thyroid, lung, colon, and pancreatic cancers.. To investigate the biological functions of Ptprj, we have generated mice deficient in this receptor protein tyrosine phosphatase.



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